Yes! Many organisations already have MXDs that define the symbology for their geospatial datasets. They have a single MXD that adheres to their corporate branding or meets legislative requirements.

If you have an MXD like this, it doesn’t need to be broken up into individual MXDs for DUT. Instead you can:

• upload it once to the first dataset you publish through DUT,
• email the team and advise which datasets it was attached to, and which other datasets contains the same symbology.

Reference: For more information see  MXDs with multiple layers and corporate styling MXDs